Estimating the density of fluorescent nanoparticles in the primo vessels in the fourth ventricle and the spinal cord of a rat.
نویسندگان
چکیده
The primo vascular system is a novel circulatory system forming a network throughout an animal's body. Primo vessels were recently observed in the fourth ventricle of the brain and in the spinal cord of a rat by using fluorescent nanoparticles. In order to quantify the nanoparticles in the primo vessels, we measured the florescence of the nanoparticles and calibrated the measurements by using a reference suspension. We removed the noise due to autofluorescence with the technique of multispectral imaging. The line densities of nanoparticles and the contrast values of their images were, respectively, 0.5 ± 0.5 ng/mm and 0.7 ± 0.5 for primo vessels in the fourth ventricle, and 1.3 ± 0.6 ng/mm and 1.4 ± 0.2 for primo vessels in the spinal cord. The data obtained from and the procedures used in this work could be useful in evaluating the feasibility of using nanoparticles as a contrast agent during MRI or CT imaging of primo vessels in the brain or the spinal cord.
منابع مشابه
A Rare Cause of Obstructive Chronic Hydrocephalus in an Adult Patient: A Case Report of Fourth Ventricle\'s Foramina Idiopathic Stenosis
Background & Importance: Idiopathic stenosis of the foramina of Magendie and Luschka is a rare cause of obstructive hydrocephalus involving the fourth ventricle. Case Presentation: We reported the case of a 40-year-old woman who developed headaches and vertigo for several months and more recently gait disturbance. The CT scan showed quadri-ventricular hydrocephalus involving mainly...
متن کاملMicroglial Activation in Rat Experimental Spinal Cord Injury Model
Background: The present study was designed to evaluate the secondary microglial activation processes after spinal cord injury (SCI). Methods: A quantitative histological study was performed to determine ED-1 positive cells, glial cell density, and cavitation size in untreated SCI rats at days 1, 2, and 4, and weeks 1, 2, 3, and 4. Results: The results of glial cell quantification along the 4900...
متن کاملStudy of the Effects of Horsetail Stem Extract Administration on the Numerical Density of Alpha Motoneurons in the Ventral Horn of Lumbar Spinal Cord of Compressed Sciatic Nerve of Rat
Purpose: The goal of this research was to study of the protective effects horse tail (HT) plant extract on the central degeneration of alpha motoneurons (AM) of spinal cord.Materials and Methods: The lesioned sciatic rats were divided into sham, control and 3 experimental groups and treated by 15 mg/kg, ip HT extract. The animals were care for one month and then were sacrificed and perfused car...
متن کاملP35: Effect of Hydroalcoholic Extract of Agrimonia eupatoria on Alpha Motoneurons Regeneration of Anterior Spinal Cord after Compression of Sciatic Nerve in Rat
If nerve cells damaged, they cannot be restored by themselves. Agrimonia eupatoria has been used in traditional medicine to heal the wounds and scratch, and dry the scars. Therefore, this herb probably contains compounds with restorative properties. The purpose of this study was to investigate the restorative effect of Agrimonia eupatoria on alpha motor neurons of anterior spinal cord. In this ...
متن کاملTransplanting P75-Suppressed Bone Marrow Stromal Cells Promotes Functional Behavior in a Rat Model of Spinal Cord Injury
Background: Bone marrow stromal cells (BMSC) have been successfully employed for movement deficit recovery in spinal cord injury (SCI) rat models. One of the unsettled problems in cell transplantation is the relative high proportion of cell death, specifically after neural differentiation. According to our previous studies, p75 receptor, known as the death receptor, is only expressed in BMSC in...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of biomedical optics
دوره 16 11 شماره
صفحات -
تاریخ انتشار 2011